Quantifying three-dimensional mouse eye rotations, especially torsion, often requires invasive implanted coils or ocular markers. We developed and validated a markerless stereo vision-based method to measure yaw, pitch, and roll eye rotations. NEW
No actionable change for clinical practice — this is a laboratory measurement tool validated in mice, not yet applicable to human vestibular assessment.
A non-invasive, markerless 3D eye-movement tracking method could accelerate preclinical vestibular research by removing barriers associated with implanted coil systems.
- 01Markerless stereo-vision system tracks 3D mouse eye movements including torsional rotation.
- 02Eliminates need for surgically implanted scleral coils or ocular markers.
- 03Validated against existing methods in a preclinical (mouse) model.
- 04Potential to improve throughput and welfare in vestibular research animal studies.
- 05Published in Journal of Neuroscience Methods; currently a research tool, not clinical.
The markerless stereo-vision method can accurately measure 3D mouse eye movements, including torsion, without implanted coils or ocular markers.
studysupported- PMID
- 42155894
- DOI
- 10.1016/j.jneumeth.2026.110807.
- Journal
- Journal of Neuroscience Methods
- Publication type
- research_article
- Evidence level
- 4
- Population
- Laboratory mice
- Intervention
- Markerless stereo-vision 3D eye-movement tracking system
- Comparator
- Conventional implanted coil or ocular marker methods
Primary outcomes
Accuracy of 3D eye movement measurement including torsion; Feasibility without invasive implants